E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain HT414

• CGSC#: 6856
• Strain Designation

  Designation	Source	Preference
  HT414	Tabor H.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 10 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  fhuA21	3.61	1	fhuA21
  lacY1	7.79	1	lacY1
  glnX44(AS)	14.99	1	glnX44(AS)
  LAM-	17.40	1	λ-
  DE(speB-speA)97	66.39	1	Δ(speB-speA)97
  DE(speC-glcB)63	66.92	1	Δ(speC-glcB)63
  rplL9(L?)	90.06	1	rplL9(L?)
  thiE1	90.34	1	thiE1
  hsdS1	98.67	1	hsdS1

  
  
• Comments:
  • The rpsL allele is important; it makes this strain the only good Spe^- strain.
• References:
  • Tabor, H., C.W. Tabor, M.S. Cohn, E.W. Hafner 1981. Streptomycin resistance (rpsL) produces an absolute requirement for polyamines for growth of an Escherichia coli strain unable to synthesize putrescine and spermidine [delta(speA-speB) delta specC]. J
  
  

  Printable Strain Report