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Strain SK7772

• CGSC#: 7289
• Strain Designation

  Designation	Source	Preference
  SK7772	Kushner S.R.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 15 Mutations:

  Mutation	Location	Certainty	Display
  DE(lacY-lacZ)286	7.78	1	Δ(lacY-lacZ)286
  tsx-3	9.28	0	tsx-3
  glnX44(AS)	14.99	1	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  LAM-	17.40	1	λ-
  lacZ95=phi80dIIlacZ9	28.00	1	lacZ95=phi80dIIlacZ9
  hisG4(Oc)	45.01	1	hisG4(Oc)
  rfbC1	45.43	0	rfbC1
  xyl-7	80.34	1	xyl-7
  mtl-1	81.26	1	mtl-1
  ilvD188(Oc)	85.17	1	ilvD188(Oc)
  uvrD291(del-ins,::tet)	86.13	1	uvrD291(del-ins,::tet)
  metE40	86.45	1	metE40
  argH1(del)	89.55	1	ΔargH1
  thiE1	90.34	0	thiE1

  
  
• Comments:
  • This is a recombination indicator strain, the lac mutations recombining to give Lac⁺ at high rates.
• References:
  • Washburn, B.K., S.R. Kushner 1991. Construction and analysis of deletions in the structural gene (uvrD) for DNA helicase II of Escherichia coli. J.Bacteriol. 173:2569-2575
  
  

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