E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain EM134

• CGSC#: 4172
• Strain Designation

  Designation	Source	Preference
  EM134	Murgola E.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 12 Mutations:

  Mutation	Location	Certainty	Display
  araC14	1.52	1	araC14
  lacY1	7.79	1	lacY1
  tsx-57	9.28	1	tsx-57
  glnX44(AS)	14.99	1	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  rpsL20(strR)	74.84	1	rpsL20(strR)
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1
  thiE1	90.34	1	thiE1
  tfr-5	1000.00	1	tfr-5
  Ssl-134	1000.00	1	Ssl-134
  ilv-275	1000.00	1	ilv-275

  
  
• Comments:
  • In Lyophil Only
  • This strain must be grown in the presence of 200γ/ml streptomycin in order to prevent selection of faster-growing revertants of the streptomycin-suppressible lethal mutation Ssl-134.
• References:
  • Murgola, EJ, EA Adelberg 1970. Streptomycin-suppressible lethal mutations in Escherichia coli. J. Bacteriol. 103(1):20-6.
  
  

  Printable Strain Report