E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain DH5α/pBAD18-Amp

• CGSC#: 12520
• Strain Designation

  Designation	Source	Preference
  DH5α/pBAD18-Amp	Beckwith J.R.	1
  DH5alpha/pBAD18-Amp		2
  pBAD18-Amp		3

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 11 Mutations:

  Mutation	Location	Certainty	Display
  DE(argF-lac)169	6.21	1	Δ(argF-lac)169
  phi80dlacZ58(M15)	8.00	1	φ80dlacZ58(M15)
  glnX44(AS)	14.99	1	glnX44(AS)
  LAM-	17.40	1	λ-
  rfbC1	45.43	1	rfbC1
  gyrA96(NalR)	50.33	1	gyrA96(NalR)
  recA1	60.80	1	recA1
  endA1	66.57	1	endA1
  spoT1	82.34	1	spoT1
  thiE1	90.34	1	thiE1
  hsdR17	98.74	1	hsdR17

  
  
• Comments:
• Episome/Plasmid: pBAD18-Amp

  Plasmid Markers/Mutations:
  ori pMB1
  araC
  bla

• References:
  • Guzman, LM, D Belin, MJ Carson, J Beckwith 1995. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177(14):4121-30.
  
  

  Printable Strain Report