E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain NK5549

• CGSC#: 6171
• Strain Designation

  Designation	Source	Preference
  NK5549	Kleckner N.	1

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 6 Mutations:

  Mutation	Location	Certainty	Display
  ara-600	1.40	1	ara-600
  DE(gpt-lac)5	5.51	1	Δ(gpt-lac)5
  relA1	62.71	0	relA1
  spoT1	82.34	0	spoT1
  rpoB0(rifR)	90.08	1	rpoB0(rifR)
  thiE1	90.34	1	thiE1

  
  
• Comments:
• Episome/Plasmid: F42-12

  Plasmid Markers/Mutations:
  lacI22
  lacZ4502::Tn10

• References:
  • Foster, TJ 1977. Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12. Mol. Gen. Genet. 154(3):305-9.
  
  

  Printable Strain Report