CGSC Strain#: 6171
Strain Designation: NK5549      Source of Strain: N. Kleckner
Sex: F' Episome/Plasmid: F42-12
   Plasmid Markers/Mutations: lacI22, lacZ4502::Tn10
Chromosomal Markers: ara-600, Δ(gpt-lac)5, relA1, spoT1, rpoB0(rifR), thiE1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• rifR = rifamycin resistance
• = tetracycline resistant

References:

• Foster, TJ 1977. Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12. Mol. Gen. Genet. 154(3):305-9.