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CGSC, The Coli Genetic Stock Center

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Strain EB34

• CGSC#: 5908
• Strain Designation

  Designation	Source	Preference
  EB34	Birge E.A.	1

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 12
• PO Map Position: 68.80
• Direction of Marker Transfer: Clockwise
• 11 Mutations:

  Mutation	Location	Certainty	Display
  DE(gpt-proA)62	5.51	1	Δ(gpt-proA)62
  lacY1 or lacZ4	8.00	1	lacY1 or lacZ4
  glnX44(AS)	14.99	0	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  LAM-	17.40	1	λ-
  rpsD2001	74.12	1	rpsD2001
  rpsE2126(SpcR)	74.20	1	rpsE2126(SpcR)
  rpsL252(strR)	74.84	1	rpsL252(strR)
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • This strain is streptomycin and spectinomycin-sensitive, presumably due to suppression of the rpsE126(SpcR) locus by rpsD2001, as well as suppression of strep-dependence of the parent strain. (See Ref.below.)
• References:
  • Birge, EA, CG Kurland 1970. Reversion of a streptomycin-dependent strain of Escherichia coli. Mol. Gen. Genet. 109(4):356-69.
  
  

  Printable Strain Report