E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain NH5033

• CGSC#: 5832
• Strain Designation

  Designation	Source	Preference
  NH5033	Howard-Flanders P.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 9 Mutations:

  Mutation	Location	Certainty	Display
  lac-61	7.77	1	lac-61
  glnX44(AS)	14.99	1	glnX44(AS)
  gal-44	17.00	1	gal-44
  LAM-	17.40	1	λ-
  sbcB15	44.85	1	sbcB15
  recB21	63.60	1	recB21
  endA1	66.57	1	endA1
  thiE1	90.34	1	thiE1
  deo-27 or deo-43	99.00	1	deo-27 or deo-43

  
  
• Comments:
  • Not a pure K-12 strain.
  • This strain is φX-sensitive but plates φX poorly with turbid plaques. This is an E. coli K-12 x C hybrid.
• References:
  • Jeng, TW, RA Hendon, H Fraenkel-Conrat 1978. Search for relationships among the hemolytic, phospholipolytic, and neurotoxic activities of snake venoms. Proc. Natl. Acad. Sci. U.S.A. 75(2):600-4.
  
  

  Printable Strain Report