CGSC Strain#: 5832
Strain Designation: NH5033      Source of Strain: P. Howard-Flanders
Sex: F- Chromosomal Markers: lac-61, glnX44(AS), gal-44, λ^-, sbcB15, recB21, endA1, thiE1, deo-27 or deo-43
Strain Comments:

• This strain is φX-sensitive but plates φX poorly with turbid plaques. This is an E. coli K-12 x C hybrid.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor

References:

• Jeng, TW, RA Hendon, H Fraenkel-Conrat 1978. Search for relationships among the hemolytic, phospholipolytic, and neurotoxic activities of snake venoms. Proc. Natl. Acad. Sci. U.S.A. 75(2):600-4.