E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain TA500

• CGSC#: 12263
• Strain Designation

  Designation	Source	Preference
  TA500	Squires C.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 8 Mutations:

  Mutation	Location	Certainty	Display
  ara-600	1.40	1	ara-600
  DE(rrsH-rrlH)770	4.82	1	Δ(rrsH-rrlH)770
  DE(codB-lacI)3	7.60	1	Δ(codB-lacI)3
  DE(rrlG-rrsG)772::lacZ	58.72	1	Δ(rrlG-rrsG)772::lacZ
  DE(rrsA-rrlA)771	86.93	1	Δ(rrsA-rrlA)771
  DE(rrsB-rrlB)769	89.76	1	Δ(rrsB-rrlB)769
  thiE1	90.34	0	thiE1
  DE(purD-metA)768	90.58	1	Δ(purD-metA)768

  
  
• Comments:
• Episome/Plasmid: pTRNA65

  Plasmid Markers/Mutations:
  ori p15A
  aadA(strR,SpcR)
  trpT
  thrV
  ileU
  aspT
  alaU
  gltT

• References:
  • Asai, T, C Condon, J Voulgaris, D Zaporojets, B Shen, M Al-Omar, C Squires, CL Squires 1999. Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons. J. Bacteriol. 181(12):3803-9.
  • Asai, T, D Zaporojets, C Squires, CL Squires 1999. An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria. Proc. Natl. Acad. Sci. U.S.A. 96(5):1971-6.
  
  

  Printable Strain Report