E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pTRNA65

• Name: pTRNA65
• Type: Plasmid
  SubSites:

  trpT

  thrV

  ileU

  aspT

  alaU

  gltT

  
  
  Plasmid Sites:

  ori p15A

  aadA(strR,SpcR)

  
  
• Priority: 0
• Comment:
• References:
  • Asai, T, D Zaporojets, C Squires, CL Squires 1999. An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria. Proc. Natl. Acad. Sci. U.S.A. 96(5):1971-6.
  
  
• 3 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  TA500	8	F-, ara-600, Δ(rrsH-rrlH)770, Δ(codB-lacI)3, Δ(rrlG-rrsG)772::lacZ, Δ(rrsA-rrlA)771, Δ(rrsB-rrlB)769, thiE1?, Δ(purD-metA)768, pTRNA65
  TA516	9	F-, ara-600, Δ(rrsH-rrlH)770, Δ(codB-lacI)3, Δ(rrlG-rrsG)772::lacZ, Δ(rrlD-rrsD)773::cat, Δ(rrsA-rrlA)771, Δ(rrsB-rrlB)769, thiE1?, Δ(purD-metA)768, pTRNA65
  TA520	10	F-, ara-600, Δ(rrsH-rrlH)770, Δ(codB-lacI)3, Δ(rrlG-rrsG)772::lacZ, Δ(rrlD-rrsD)773::cat, Δ(rrsA-rrlA)771, Δ(rrsB-rrlB)769, thiE1?, Δ(purD-metA)768, pTRNA65, pHK-rrnC