Strain TA527
- CGSC#: 12282
- Strain Designation
Designation Source Preference TA527 Squires C.L. 1 - Sex(Hfr,F+,F-,or F'): F-
- 10 Mutations:
Mutation Location Certainty Display ara-600 1.40 1 ara-600 DE(rrsH-rrlH)770 4.82 1 Δ(rrsH-rrlH)770 DE(codB-lacI)3 7.60 1 Δ(codB-lacI)3 DE(rrlG-rrsG)772::lacZ 58.72 1 Δ(rrlG-rrsG)772::lacZ DE(rrlD-rrsD)773::cat 73.75 1 Δ(rrlD-rrsD)773::cat DE(rrsC-rrlC)774::cat 84.96 1 Δ(rrsC-rrlC)774::cat DE(rrsA-rrlA)771 86.93 1 Δ(rrsA-rrlA)771 DE(rrsB-rrlB)769 89.76 1 Δ(rrsB-rrlB)769 thiE1 90.34 0 thiE1 DE(purD-metA)768 90.58 1 Δ(purD-metA)768 - Comments:
- Episome/Plasmid: ptRNA66
Plasmid Markers/Mutations: aadA(strR,SpcR) ori p15A trpT aspT ileU alaU thrV - Episome/Plasmid: pHK-rrnC
Plasmid Markers/Mutations: kan oriR101 trpT rrsC rrlC rrfC gltU aspT - References:
- Asai, T, C Condon, J Voulgaris, D Zaporojets, B Shen, M Al-Omar, C Squires, CL Squires 1999. Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons. J. Bacteriol. 181(12):3803-9.
- Asai, T, D Zaporojets, C Squires, CL Squires 1999. An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria. Proc. Natl. Acad. Sci. U.S.A. 96(5):1971-6.
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