E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain PP48

• CGSC#: 5362
• Strain Designation

  Designation	Source	Preference
  PP48	Perlman & Pastan	1
  5336	Perlman & Pastan	2

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 1
• PO Map Position: 96.80
• Direction of Marker Transfer: Clockwise
• 6 Mutations:

  Mutation	Location	Certainty	Display
  LAM-	17.40	1	λ-
  e14-	26.00	1	e14-
  relA1	62.71	1	relA1
  cyaA1	85.98	1	cyaA1
  thiE1	90.34	1	thiE1
  bglR11	1000.00	1	bglR11

  
  
• Comments:
  • The strain must be purified frequently by streaking on MacConkey-Lac and selecting Lac^- colonies.
• References:
  • Perlman, RL, I Pastan 1969. Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coli. Biochem. Biophys. Res. Commun. 37(1):151-7.
  
  

  Printable Strain Report