CGSC Strain#: 5362
Strain Designation: PP48      Source of Strain: Perlman & Pastan
Other Designations: 5336
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, relA1, cyaA1, thiE1, bglR11
Strain Comments:

• The strain must be purified frequently by streaking on MacConkey-Lac and selecting Lac^- colonies.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• cyaA1-- marker is very unstable and the strain should be checked for reversion. The marker comes from Pasten's strain 5336.
• thiE1 was formerly called thi-1

References:

• Perlman, RL, I Pastan 1969. Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coli. Biochem. Biophys. Res. Commun. 37(1):151-7.