Strain KL709
- CGSC#: 4279
- Strain Designation
Designation Source Preference KL709 Cappello M.C. 1 KLF42 2 - Sex(Hfr,F+,F-,or F'): F'
- 13 Mutations:
Mutation Location Certainty Display galK35 16.99 1 galK35 LAM- 17.40 1 λ- pyrD34 21.64 1 pyrD34 e14- 26.00 1 e14- trpC45 28.38 1 trpC45 his-68 45.01 1 his-68 tyrA2 59.00 1 tyrA2 recA1 60.80 1 recA1 rpsL118(strR) 74.84 1 rpsL118(strR) malT1(LamR) 76.54 1 malT1(λR) xyl-7 80.34 1 xyl-7 mtlA2 81.26 1 mtlA2 thiE1 90.34 1 thiE1 - Comments:
- This strain should be maintained on medium containing thi, his, trp, ura and lacking tyr. Watch out for faster-growing clones bearing further partial deletions of F142.
- Episome/Plasmid: F142
Plasmid Markers/Mutations: ptsI0(del) - References:
- Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.
- Gelfand, D.H., N. Rudo 1977. Mapping of the aspartate and aromatic amino acid amino-transferase genes tyrB and aspC. J.Bacteriol. 130:441-444
- Gelfand, DH, RA Steinberg 1977. Escherichia coli mutants deficient in the aspartate and aromatic amino acid aminotransferases. J. Bacteriol. 130(1):429-40.
Printable Strain Report