CGSC Strain#: 4279
Strain Designation: KL709      Source of Strain: M.C. Cappello
Other Designations: KLF42
Sex: F' Episome/Plasmid: F142
   Plasmid Markers/Mutations: ΔptsI0
Chromosomal Markers: galK35, λ^-, pyrD34, e14-, trpC45, his-68, tyrA2, recA1, rpsL118(strR), malT1(λ^R), xyl-7, mtlA2, thiE1
Strain Comments:

• This strain should be maintained on medium containing thi, his, trp, ura and lacking tyr. Watch out for faster-growing clones bearing further partial deletions of F142.
• galK35-- is Brenner's gal^-_U99 from Hfr 3000 U99 and has been called galK99
• e14--- : Loss of the e14 element makes the strain McrA^-.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• LamR = Lambda-resistant
• strR = streptomycin resist.

References:

• Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.
• Gelfand, D.H., N. Rudo 1977. Mapping of the aspartate and aromatic amino acid amino-transferase genes tyrB and aspC. J.Bacteriol. 130:441-444
• Gelfand, DH, RA Steinberg 1977. Escherichia coli mutants deficient in the aspartate and aromatic amino acid aminotransferases. J. Bacteriol. 130(1):429-40.