E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain KL414

• CGSC#: 4377
• Strain Designation

  Designation	Source	Preference
  KL414	Cappello M.C.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 14 Mutations:

  Mutation	Location	Certainty	Display
  araC14	1.52	1	araC14
  leuB6(Am)	1.74	1	leuB6(Am)
  lacZ36	7.81	1	lacZ36
  proC32	8.71	1	proC32
  LAM-	17.40	1	λ-
  hisF860(Stable)	45.13	1	hisF860(Stable)
  recA56	60.80	1	recA56
  thyA54	63.86	1	thyA54
  rpsE2115(SpcR)	74.20	1	rpsE2115(SpcR)
  rpsL109(strR)	74.84	1	rpsL109(strR)
  xylA5	80.34	1	xylA5
  mtlA2	81.26	1	mtlA2
  polA12(ts)	87.18	1	polA12(ts)
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • This strain may carry malA38 and sup-78, an uncharacterized mutation near his which confers a Mal⁺ phenotype.
  • This strain was originally grown in LB with 0.5% salt, thymine should also be added
• References:
  • Lloyd, R.G., K.B. Low, G.N. Godson, E.A. Birge 1974. Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive RecA- phenotype. J.Bacteriol. 120:407-415
  
  

  Printable Strain Report