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Mutation polA12(ts)

• Name: polA12(ts)
• Type: Unknown
• Mutation of Gene: polA
• Approx. Map Location: 87.18
• Properties

  Property	Symbol
  Temperature Sensitive	ts

  
  
• Comments
  • : sensitive to temperature and to low salt; altered pH optimum. Normal Exo levels. Totally defective in nick translation at 42C. recA56 polA12 are ts for growth.
• References
  • Uyemura, D, DC Eichler, IR Lehman 1976. Biochemical characterization of mutant forms of DNA polymerase I from Escherichia coli. II. The polAex1 mutation. J. Biol. Chem. 251(13):4085-9.
  • Grindley, ND, WS Kelley 1976. Effects of different alleles of the E. coli K12 pol A gene on the replication of non-transferring plasmids. Mol. Gen. Genet. 143(3):311-8.
• 10 Strains Carrying This Mutation

  Name	Mutations	Genotype
  ES1	4	F-, lacY1 or lacZ53(Am), uvrC34, rpsL31(strR) or rpsL151(strR), polA12(ts)
  AB6007	7	F-, lacY1, uvrC34, thyA50, rpsL31(strR), rpsL151(strR), polA12(ts), deoC8
  ES2	7	F-, lacZ53(Am), λ-, IN(rrnD-rrnE)1, rpsL151(strR), polA12(ts), rha-5, uvrA6
  MM386	7	F-, lacZ53(Am), λ-, relA1?, rpsL151(strR), polA12(ts), rha-5, deoC2?
  MM383	8	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), polA12(ts), rha-5, deoC2
  MM387	8	F-, lacZ53(Am), λ-, relA1?, recB21, rpsL151(strR), polA12(ts), rha-5, deoC2
  MM385	9	F-, lacZ53(Am), λ-, recA56, relA1?, IN(rrnD-rrnE)1, rpsL151(strR), polA12(ts), rha-5, deoC2?
  KL405	13	F-, araC14, leuB6(Am), lacZ36, proC32, hisF860(Stable), thyA54, rpsE2115(SpcR), rpsL109(strR), malA38, xylA5, mtl-1, polA12(ts), thiE1
  KL413	14	F-, araC14, leuB6(Am), lacZ36, proC32, hisF860(Stable), recA200(ts), thyA54, rpsE2115(SpcR), rpsL109(strR), malA38, xylA5, mtl-1, polA12(ts), thiE1
  KL414	14	F-, araC14, leuB6(Am), lacZ36, proC32, λ-, hisF860(Stable), recA56, thyA54, rpsE2115(SpcR), rpsL109(strR), xylA5, mtlA2, polA12(ts), thiE1