E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain KL399

• CGSC#: 4359
• Strain Designation

  Designation	Source	Preference
  KL399	Cappello M.C.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 16 Mutations:

  Mutation	Location	Certainty	Display
  araC14	1.52	1	araC14
  leuB6(Am)	1.74	1	leuB6(Am)
  lacZ36	7.81	1	lacZ36
  proC32	8.71	1	proC32
  LAM-	17.40	1	λ-
  hisF860(Stable)	45.13	1	hisF860(Stable)
  recA200(ts)	60.80	1	recA200(ts)
  thyA54	63.86	1	thyA54
  rpsE2115(SpcR)	74.20	1	rpsE2115(SpcR)
  rpsL109(strR)	74.84	1	rpsL109(strR)
  malA38	76.50	1	malA38
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1
  metE70	86.45	1	metE70
  thiE1	90.34	1	thiE1
  deo-85	99.40	1	deo-85

  
  
• Comments:
  • The RecA(Ts) phenotype can be demonstrated only on a low-salt medium (0.5g per liter). This strain carries an uncharacterized mutation near his which confers a Mal⁺ phenotype.
• References:
  • Lloyd, R.G., K.B. Low, G.N. Godson, E.A. Birge 1974. Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive RecA- phenotype. J.Bacteriol. 120:407-415
  
  

  Printable Strain Report