E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain EDCM25

• CGSC#: 8016
• Strain Designation

  Designation	Source	Preference
  EDCM25	Masters M.	1
  DH5alpha/pTOF2		2

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 11 Mutations:

  Mutation	Location	Certainty	Display
  DE(argF-lac)169	6.21	1	Δ(argF-lac)169
  phi80dlacZ58(M15)	8.00	1	φ80dlacZ58(M15)
  glnX44(AS)	14.99	1	glnX44(AS)
  LAM-	17.40	1	λ-
  rfbC1	45.43	0	rfbC1
  gyrA96(NalR)	50.33	1	gyrA96(NalR)
  recA1	60.80	1	recA1
  endA1	66.57	1	endA1
  spoT1	82.34	0	spoT1
  thiE1	90.34	1	thiE1
  hsdR17	98.74	1	hsdR17

  
  
• Comments:
• Episome/Plasmid: pTOF2

  Plasmid Markers/Mutations:
  bla(ApR)
  FRT-aph(kanR)-FRT2
  FRT-aph(kanR)-FRT

• References:
  • Merlin, C., S. McAteer, M. Masters 2002. Tools for characterization of Escherichia coli genes of unknown function. J.Bacteriol. 184:4573-4581
  
  

  Printable Strain Report