E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pTOF2

• Name: pTOF2
• Type: Plasmid
  SubSites:

  FRT-aph(kanR)-FRT

  
  
  Plasmid Sites:

  bla(ApR)

  FRT-aph(kanR)-FRT2

  
  
• Priority: 0
• Comment:
  • FRT-aph(kanR)-FRT = FK2 cassette of Merlin et al. Same as FK1, but aph gene in opposite orientation.
• References:
  • Merlin, C., S. McAteer, M. Masters 2002. Tools for characterization of Escherichia coli genes of unknown function. J.Bacteriol. 184:4573-4581
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  EDCM25	11	F-, Δ(argF-lac)169, φ80dlacZ58(M15), glnX44(AS), λ-, rfbC1?, gyrA96(NalR), recA1, endA1, spoT1?, thiE1, hsdR17, pTOF2