E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain CR34 Thy- SR

• CGSC#: 6707
• Strain Designation

  Designation	Source	Preference
  CR34 Thy- SR	Gross J.	1
  JW116	Wechsler J.A.	2

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 13 Mutations:

  Mutation	Location	Certainty	Display
  thr-1	0.01	1	thr-1
  leuB6(Am)	1.74	1	leuB6(Am)
  fhuA21	3.61	1	fhuA21
  cyn-101	7.72	1	cyn-101
  lacY1	7.79	1	lacY1
  glnX44(AS)	14.99	1	glnX44(AS)
  LAM-	17.40	1	λ-
  rfbC1	45.43	1	rfbC1
  thyA6	63.86	1	thyA6
  rpsL67(strR)	74.84	1	rpsL67(strR)
  glpR200(glp$^c$)	76.68	0	glpR200(glpc)
  thiE1	90.34	1	thiE1
  deoC1	99.48	1	deoC1

  
  
• Comments:
  • Ref. to parent strain CR34 Thy^-.
• References:
  • Okada, T., K. Yanagisawa, A. Ryan 1961. Z.Vererbungsl. 92:402-0
  • Wechsler, JA, JD Gross 1971. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol. Gen. Genet. 113(3):273-84.
  • Rooney, SA, H Goldfine 1972. Isolation and characterization of 2-keto-3-deoxyoctonate-lipid A from a heptose-deficient mutant of Escherichia coli. J. Bacteriol. 111(2):531-41.
  • Koplow, J, H Goldfine 1974. Alterations in the outer membrane of the cell envelope of heptose-deficient mutants of Escherichia coli. J. Bacteriol. 117(2):527-43.
  • Coleman, WG, KS Deshpande 1985. New cysE-pyrE-linked rfa mutation in Escherichia coli K-12 that results in a heptoseless lipopolysaccharide. J. Bacteriol. 161(3):1209-14.
  
  

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