Strain MA-1
- CGSC#: 5624
- Strain Designation
Designation Source Preference MA-1 Broekman & Hoekstra 1 PC1978 2 - Sex(Hfr,F+,F-,or F'): F-
- 17 Mutations:
Mutation Location Certainty Display thr-1 0.01 1 thr-1 leuB6(Am) 1.74 1 leuB6(Am) fhuA21 3.61 1 fhuA21 codA1 7.66 1 codA1 lacY1 7.79 1 lacY1 tsx-95 9.28 1 tsx-95 glnX44(AS) 14.99 1 glnX44(AS) LAM- 17.40 1 λ- pyrF101 28.88 1 pyrF101 his-108 44.00 1 his-108 fabB3 52.56 1 fabB3 thyA6 63.86 1 thyA6 argG66 71.48 1 argG66 rpsL201(strR) 74.84 1 rpsL201(strR) ilvA634 85.21 1 ilvA634 thiE1 90.34 1 thiE1 deoC1 99.48 1 deoC1 - Comments:
- This strain requires 0.01% oleate added to Luria medium ( fabB-). Since fatty acid uptake is subject to catabolite repression, we use succinate+oleate+Brij 58 in minimal and Luria.
- References:
- Broekman, J.H.F.F., W.P.M. Hoekstra 1973. Mapping of fabC, a locus for the biosynthesis of unsaturated fatty acids in Escherichia coli K12. Mol.Gen.Genet. 124:65-0
- Clark, D, JE Cronan 1977. Further mapping of several membrane lipid biosynthetic genes (fabC, fabB, gpsA, plsB) of Escherichia coli. J. Bacteriol. 132(2):549-54.
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