E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain F500/GMS724

• CGSC#: 5505
• Strain Designation

  Designation	Source	Preference
  F500/GMS724	Novel G.	1

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 11 Mutations:

  Mutation	Location	Certainty	Display
  lacY1	7.79	1	lacY1
  tsx-29	9.28	0	tsx-29
  glnX44(AS)	14.99	0	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  LAM-	17.40	1	λ-
  manA4	36.35	1	manA4
  aroD6	38.21	1	aroD6
  recA1	60.80	1	recA1
  rpsL700(strR)	74.84	1	rpsL700(strR)
  mtl-1	81.26	1	mtl-1
  metB1	88.94	1	metB1

  
  
• Comments:
  • This strain must be maintained on minimal medium with mannose as carbon source, containing Met and lacking aromatic amino acids.
• Episome/Plasmid: F500

  Plasmid Markers/Mutations:

• References:
  • Novel, M., G. Novel 1976. Regulation of beta-glucuronidase synthesis in Escherichia coli K-12: constitutive mutants specifically derepressed for uidA expression. J.Bacteriol. 127:406-417
  
  

  Printable Strain Report