CGSC Strain#: 5505
Strain Designation: F500/GMS724      Source of Strain: G. Novel
Sex: F' Episome/Plasmid: F500
   Plasmid Markers/Mutations:
Chromosomal Markers: lacY1, tsx-29, glnX44(AS), galK2(Oc), λ^-, manA4, aroD6, recA1, rpsL700(strR), mtl-1, metB1
Strain Comments:

• This strain must be maintained on minimal medium with mannose as carbon source, containing Met and lacking aromatic amino acids.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Novel, M., G. Novel 1976. Regulation of beta-glucuronidase synthesis in Escherichia coli K-12: constitutive mutants specifically derepressed for uidA expression. J.Bacteriol. 127:406-417