E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain BW23474/pAH70

• CGSC#: 7845
• Strain Designation

  Designation	Source	Preference
  BW23474/pAH70	Wanner B.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 9 Mutations:

  Mutation	Location	Certainty	Display
  DE(argF-lac)169	6.21	1	Δ(argF-lac)169
  uidA4(del)::pir-116	36.48	1	ΔuidA4::pir-116
  recA1	60.80	1	recA1
  rpoS396(Am)	61.75	0	rpoS396(Am)
  endA9(del-ins)::FRT	66.57	1	endA9(del-ins)::FRT
  rph-1	82.20	1	rph-1
  hsdR514	98.74	1	hsdR514
  rob-1	99.85	1	rob-1
  creC510	99.89	1	creC510

  
  
• Comments:
• Episome/Plasmid: pAH70

  Plasmid Markers/Mutations:
  oriR6Kgamma
  Phi Psyn1-pstS*
  tL3LAM(Ter)
  HK022(att)
  rgnB(Ter)
  kan
  tL3$\_{lambda}$
  attHK022
  oriRg
  kan
  rgnB

• References:
  • Haldimann, A, BL Wanner 2001. Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. J. Bacteriol. 183(21):6384-93.
  
  

  Printable Strain Report