E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain K8-5m

• CGSC#: 4868
• Strain Designation

  Designation	Source	Preference
  K8-5m	Kornberg H.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 9 Mutations:

  Mutation	Location	Certainty	Display
  lacY1 or lacZ4	8.00	1	lacY1 or lacZ4
  tsx-6	9.28	0	tsx-6
  glnX44(AS)	14.99	0	glnX44(AS)
  galK2(Oc)	16.99	1	galK2(Oc)
  rpsL159(strR)	74.84	1	rpsL159(strR)
  xyl-7 or xylA5	80.30	1	xyl-7 or xylA5
  metF24	89.03	1	metF24
  aceA3	90.85	1	aceA3
  iclR13	90.97	1	iclR13

  
  
• Comments:
  • Kornberg reports that the met marker is metB or metF. iclR13 was discovered in this strain by Vinopal & Fraenkel.
• References:
  • Vinopal, RT, DG Fraenkel 1974. Phenotypic suppression of phosphofructokinase mutations in Escherichia coli by constitutive expression of the glyoxylate shunt. J. Bacteriol. 118(3):1090-100.
  
  

  Printable Strain Report