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Strain BW31004

• CGSC#: 8184
• Strain Designation

  Designation	Source	Preference
  BW31004	Wanner B.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 6 Mutations:

  Mutation	Location	Certainty	Display
  DE(araD-araB)567	1.40	1	Δ(araD-araB)567
  lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
  LAM-	17.40	1	λ-
  att80(CmR)::LZ43rhaBp-GFP	28.20	1	att80(CmR)::LZ43rhaBp-GFP
  DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
  hsdR514	98.74	1	hsdR514

  
  
• Comments:
  • Insertion of pLZ43 at att_φ80 site, resulting in GFP on the chromosome, inducible by rhamnose. Also CmR from pLZ43 is present.
• References:
  • Zhou, L., K. Zhang, B.L. Wanner 2004. Chromosomal expression of foreign and native genes from regulatable promoters in Escherichia coli. Methods Mol Biol 267:123-134
  
  

  Printable Strain Report