E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain BW27535

• CGSC#: 8174
• Strain Designation

  Designation	Source	Preference
  BW27535	Wanner B.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 8 Mutations:

  Mutation	Location	Certainty	Display
  DE(araD-araB)567	1.40	1	Δ(araD-araB)567
  lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
  LAM-	17.40	1	λ-
  araEp-531(del)::kan	64.20	1	ΔaraEp-531::kan
  Phi P_cp13 araE534	64.21	1	φPcp13araE534
  rph-1	82.20	1	rph-1
  DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
  hsdR514	98.74	1	hsdR514

  
  
• Comments:
• References:
  • Khlebnikov, A., K.A. Datsenko, T. Skaug, B.L. Wanner, J.D. Keasling 2001. Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiol 147:3241-3247
  
  

  Printable Strain Report