Strain - TK2474/pAC-Cys(AS)

Strain - TK2474/pAC-Cys(AS)Strain TK2474/pAC-Cys(AS)

Strain TK2474/pAC-Cys(AS)

CGSC#: 7906
Strain Designation
Designation Source Preference
TK2474/pAC-Cys(AS) Epstein W. 1
Sex(Hfr,F+,F-,or F'): F-

Designation	Source	Preference
TK2474/pAC-Cys(AS)	Epstein W.	1

8 Mutations:

Mutation	Location	Certainty	Display
lacZ82(Am)	7.81	1	lacZ82(Am)
nagA1	15.11	1	nagA1
DE(kdpC-kdpA)18	15.59	1	Δ(kdpC-kdpA)18
endA-	66.50	1	endA-
trkA405	74.02	1	trkA405
kup-1	84.69	1	kup-1
argE-(Am)	90.00	1	argE-(Am)
thiE1	90.34	1	thiE1

Comments:
- 1.Please note this correction on a detail of the plasmid description in reference below. The plasmid was cut with AvaI and EcoRV, rather than EcoRI and HindIII as stated on p. 9591, for insertion of
- 2.the suppressor gene into the plasmid pACYC184.
Episome/Plasmid: pAC-Cys(AS)
Plasmid Markers/Mutations:
cysT(AS*)
cat(Am)
References:
- Dorus, S., H. Mimura, W. Epstein 2001. Substrate-binding clusters of the K+-transporting Kdp ATPase of Escherichia coli investigated by amber suppression. J.Biol.Chem. 276:9590-9598
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Printable Strain Report

Plasmid Markers/Mutations:
cysT(AS*)
cat(Am)