Strain - CJ236

Strain - CJ236Strain CJ236

Strain CJ236

CGSC#: 6793
Strain Designation
Designation Source Preference
CJ236 Joyce C.M. 1
Sex(Hfr,F+,F-,or F'): F+
6 Mutations:
Mutation Location Certainty Display
LAM- 17.40 1 λ^-
ung-1 58.52 1 ung-1
relA1 62.71 1 relA1
dut-1 82.16 1 dut-1
spoT1 82.34 1 spoT1
thiE1 90.34 1 thiE1
Comments:
- 1.This strain was constructed by mating pCJ105 into an F^- derivative of B. Weiss strain BW313. pCJ105 is an F-factor carrying a chloramphenicol resistance marker
- 2.and is a derivative of pOX38 of Guyer et al. (1981 Cold Spring Harbor Symp.Quant.Biol.45:135). The plasmid is described in the reference cited below.
- 3. The use of this strain in preparing uracil-containing M13 templates is described in Methods Enzymol. 154:367. [Please note the following: (1) Streak on LB agar plus
- 4. ca. 15 ug/ml chloramphenicol to ensure that you start with an F⁺ host. (2) Cam is much more potent in liquid culture, so beware Cam selection in liquid media.
- 5. (3) CJ236 is non-suppressing. Do not use M13 vectors that carry amber mutations (mp8,9 and some mp10,11). Pers.Comm.C.M.Joyce]
Episome/Plasmid: pCJ105
Plasmid Markers/Mutations:
F::CamR
CamR
References:
- Joyce, C.M., N. Grindley 1984. Method for determining whether a gene of Escherichia coli is essintial: application to the polA gene. J.Bacteriol. 158:636-643
Printable Strain Report

Mutation	Location	Certainty	Display
LAM-	17.40	1	λ^-
ung-1	58.52	1	ung-1
relA1	62.71	1	relA1
dut-1	82.16	1	dut-1
spoT1	82.34	1	spoT1
thiE1	90.34	1	thiE1

Plasmid Markers/Mutations:
F::CamR
CamR