Strain - DF575

Strain - DF575Strain DF575

Strain DF575

Designation	Source	Preference
DF575	Fraenkel D.	1

10 Mutations:

Mutation	Location	Certainty	Display
glnX44(AS)	14.99	1	glnX44(AS)
LAM-	17.40	1	λ^-
eno-2	62.61	1	eno-2
relA1	62.71	1	relA1
rpsL104	74.84	1	rpsL104
malA	76.50	0	malA
malT1(LamR)	76.54	0	malT1(λ^R)
xyl-7	80.34	0	xyl-7
mtlA2	81.26	0	mtlA2
thiE1	90.34	0	thiE1

Comments:
- 1.This strain can be grown in a minimal medium containing malate (25mM), glycerol (12.5 mM) and casamino acids (0.1%).It should not be grown in the presence of glucose, which selects for revertants.
- 2.Reference: Hillman and Fraenkel 1975 J.Bacteriol.122:1175
- Donor markers transferred early in the cross, but not near the selected region and not been tested for: fhuA22, garB10, creC570, mcrB1, rrnB-2, spoT1, pit-10. relA1 is a donor marker near the selected argA⁺ and very likely to be
- The markers shown with ? marks are carried by the recipient parent in regions not selected for, but introduced early by the Hfr. λ^- and glnV44 are recipient markers in a region transferred very late, so are likely to be present.
References:
- Thomson, J, PD Gerstenberger, DE Goldberg, E Gociar, A Orozco de Silva, DG Fraenkel 1979. ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt. J. Bacteriol. 137(1):502-6.
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