E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain R2D2

• CGSC#: 6052
• Strain Designation

  Designation	Source	Preference
  R2D2	Felton J.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 9 Mutations:

  Mutation	Location	Certainty	Display
  ara-55	1.40	1	ara-55
  DE(codB-lacI)3	7.60	1	Δ(codB-lacI)3
  asnB50::Tn5	15.02	1	asnB50::Tn5
  gyrA91(NalR)	50.33	1	gyrA91(NalR)
  relA1	62.71	0	relA1
  spoT1	82.34	0	spoT1
  asnA31	84.60	1	asnA31
  rpoB318	90.08	1	rpoB318
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • The rpoB allele is described as a "cryptic" mutation.
• References:
  • Felton, J, S Michaelis, A Wright 1980. Mutations in two unlinked genes are required to produce asparagine auxotrophy in Escherichia coli. J. Bacteriol. 142(1):221-8.
  
  

  Printable Strain Report