E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain FEJ-1

• CGSC#: 5061
• Strain Designation

  Designation	Source	Preference
  FEJ-1	Epstein W.	1

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 13
• PO Map Position: 84.98
• Direction of Marker Transfer: Counterclockwise
• 7 Mutations:

  Mutation	Location	Certainty	Display
  leuB6(Am)	1.74	1	leuB6(Am)
  lacZ4	7.81	1	lacZ4
  glnX44(AS)	14.99	1	glnX44(AS)
  srl-1	58.40	1	srl-1
  rpsL8	74.84	1	rpsL8
  mtlA9	81.26	1	mtlA9
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • 1.The srl marker is quite stable. Selection for Srl⁺ works better when the strains are mtl^- because sorbitol has a bit of mannitol in it. Even with
  • 2.double mutants, one obtains a bit of background growth; repurification after transduction is essential to obtain reproducible results.
• References:
  
  

  Printable Strain Report