E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain DF52

• CGSC#: 7319
• Strain Designation

  Designation	Source	Preference
  DF52		1
  GN2		3

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 1
• PO Map Position: 96.80
• Direction of Marker Transfer: Clockwise
• 8 Mutations:

  Mutation	Location	Certainty	Display
  lacI22	7.88	1	lacI22
  dctB3	16.40	1	dctB3
  LAM-	17.40	1	λ-
  glk-1	54.03	1	glk-1
  ptsI2	54.58	1	ptsI2
  relA1	62.71	1	relA1
  spoT1	82.34	1	spoT1
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • This strain was briefly called GN2
• References:
  • FRAENKEL, DG, F FALCOZ-KELLY, BL HORECKER 1964. THE UTILIZATION OF GLUCOSE 6-PHOSPHATE BY GLUCOKINASELESS AND WILD-TYPE STRAINS OF ESCHERICHIA COLI. Proc. Natl. Acad. Sci. U.S.A. 52:1207-13.
  • Tanaka, S, DG Fraenkel, EC Lin 1967. The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli. Biochem. Biophys. Res. Commun. 27(1):63-7.
  • Lo, T.C.Y., M.K. Rayman, B.D. Sanwal 1972. Transport of succinate in Escherichia coli.I. Biochemical and Genetic studies of transport in whole cells. J.Biol.Chem. 247:6323-307
  
  

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