E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain NK5648

• CGSC#: 6172
• Strain Designation

  Designation	Source	Preference
  NK5648	Kleckner N.	1

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 7 Mutations:

  Mutation	Location	Certainty	Display
  araBAD-0	1.40	1	araBAD-0
  DE(gpt-lac)5	5.51	1	Δ(gpt-lac)5
  glnX44(AS)	14.99	1	glnX44(AS)
  metB-	88.90	1	metB-
  argE-(Am)	90.00	1	argE-(Am)
  rpoB0(rifR)	90.08	0	rpoB0(rifR)
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • The ara and rpo alleles are not identified because of the unknown derivation of the strain.
  • The episome carries Tn 9 inserted into lacZ or lacY. We do not know the derivation of this strain. The F' was described to us as follows: lacI^QL8 pro with Tn9 in lacYZ
• Episome/Plasmid: F128-12

  Plasmid Markers/Mutations:
  lacIp-4000(lacIQ)
  lacZp-4008(L8)
  lac-::Tn9

• References:
  
  

  Printable Strain Report