Strain - DH5α/pRH133

Strain - DH5α/pRH133Strain DH5α/pRH133

Strain DH5α/pRH133

CGSC#: 12382
Strain Designation
Designation Source Preference
DH5α/pRH133 Stahl F. 1
pRH133 2
Sex(Hfr,F+,F-,or F'): F-

11 Mutations:

Mutation	Location	Certainty	Display
DE(argF-lac)169	6.21	1	Δ(argF-lac)169
phi80dlacZ58(M15)	8.00	1	φ80dlacZ58(M15)
glnX44(AS)	14.99	1	glnX44(AS)
LAM-	17.40	1	λ^-
rfbC1	45.43	1	rfbC1
gyrA96(NalR)	50.33	1	gyrA96(NalR)
recA1	60.80	1	recA1
endA1	66.57	1	endA1
spoT1	82.34	1	spoT1
thiE1	90.34	1	thiE1
hsdR17	98.74	1	hsdR17

Comments:
- New subculture made 12/17/12
- The pRH133 plasmid expresses the phage P1 recombinase Cre.
Episome/Plasmid: pRH133
Plasmid Markers/Mutations:
bla(ApR)
bla
ori[pMB1]
References:
- Wierzbicki, A, M Kendall, K Abremski, R Hoess 1987. A mutational analysis of the bacteriophage P1 recombinase Cre. J. Mol. Biol. 195(4):785-94.
Printable Strain Report