E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Strain BT339

• CGSC#: 12130
• Strain Designation

  Designation	Source	Preference
  BT339	Wackernagel W.	1
  DH5alpha/pCP16		2
  pCP16		3

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 11 Mutations:

  Mutation	Location	Certainty	Display
  DE(argF-lac)169	6.21	1	Δ(argF-lac)169
  phi80dlacZ58(M15)	8.00	1	φ80dlacZ58(M15)
  glnX44(AS)	14.99	1	glnX44(AS)
  LAM-	17.40	1	λ-
  rfbC1	45.43	1	rfbC1
  gyrA96(NalR)	50.33	1	gyrA96(NalR)
  recA1	60.80	1	recA1
  endA1	66.57	1	endA1
  spoT1	82.34	1	spoT1
  thiE1	90.34	1	thiE1
  hsdR17	98.74	1	hsdR17

  
  
• Comments:
• Episome/Plasmid: pCP16

  Plasmid Markers/Mutations:

• References:
  • Cherepanov, PP, W Wackernagel 1995. Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158(1):9-14.
  
  

  Printable Strain Report