Strain - MI226a

Strain - MI226aStrain MI226a

Strain MI226a

CGSC#: 5768
Strain Designation
Designation Source Preference
MI226a Iaccarino M. 1
Sex(Hfr,F+,F-,or F'): F-

12 Mutations:

Mutation	Location	Certainty	Display
araBAD-9	1.40	1	araBAD-9
fhuA1	3.61	0	fhuA1
lacZ13(Oc)	7.81	1	lacZ13(Oc)
tsx-3	9.28	1	tsx-3
LAM-	17.40	0	λ^-
trpE3(Oc)	28.44	1	trpE3(Oc)
hisG4(Oc)	45.01	1	hisG4(Oc)
rpsL8 or rplL9(L?)	73.00	1	rpsL8 or rplL9(L?)
malT1(LamR)	76.54	1	malT1(λ^R)
ilvG605(Am)	85.10	1	ilvG605(Am)
ilvG603(Act)	85.10	1	ilvG603(Act)
thiE1	90.34	1	thiE1

Comments:
- This strain is IlvG^- due to the ilvG605 mutation.
References:
- Favre, R, A Wiater, S Puppo, M Iaccarino 1976. Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12. Mol. Gen. Genet. 143(3):243-52.
- Lawther, R.P., D.H. Calhoun, J.E. Gray, C.W. Adams, C.A. Hauser, G.W. Hatfield 1982. DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12. J.Bacteriol. 149:294-298
Printable Strain Report