E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain AT997

• CGSC#: 4547
• Strain Designation

  Designation	Source	Preference
  AT997	Taylor A.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 45
• PO Map Position: 65.00
• Direction of Marker Transfer: Counterclockwise
• 6 Mutations:

  Mutation	Location	Certainty	Display
  LAM-	17.40	1	λ-
  e14-	26.00	1	e14-
  dapA15	55.98	1	dapA15
  relA1	62.71	1	relA1
  spoT1	82.34	1	spoT1
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • See also Yeh et al, 1988, MGG. The dap mutation was originally ascribed to dapC, but feedback reports characterize it as dapA.
  • This strain can be maintained on nutrient broth (or L broth) only if the medium is supplemented with 40-50 ug/ml of DL-diaminopimelic acid. This strain may carry a temperature-sensitive mutation.
• References:
  • Bukhari, A.I., A.L. Taylor 1971. Genetic analysis of diaminopimelic acid- and lysine-requiring mutants of Escherichia coli. J.Bacteriol. 105:844-854
  
  

  Printable Strain Report