Strain JC5219
- CGSC#: 4324
- Strain Designation
Designation Source Preference JC5219 Clark A.J. 1 AB1157, Su- 2 ED85 Edinburgh 2 - Sex(Hfr,F+,F-,or F'): F-
- 14 Mutations:
Mutation Location Certainty Display thr-1 0.01 1 thr-1 araC14 1.52 1 araC14 leuB6(Am) 1.74 1 leuB6(Am) DE(gpt-proA)62 5.51 1 Δ(gpt-proA)62 lacY1 7.79 1 lacY1 tsx-33 9.28 1 tsx-33 LAM- 17.40 1 λ- hisG4(Oc) 45.01 1 hisG4(Oc) rpsL31(strR) 74.84 1 rpsL31(strR) xylA5 80.34 1 xylA5 mtl-1 81.26 1 mtl-1 rha-3 88.00 1 rha-3 argE3(Oc) 89.48 1 argE3(Oc) Met-(ts) or Cys-0 1000.00 1 Met-(ts) or Cys-0 - Comments:
- This strain was intended to be a suppressor-free derivative of AB1157. After a "supE+" revertant was selected, a requirement for CYS or MET appeared.
- We have received reports that the strain still carries a suppressor of some sort. The rha marker was introduced separately.
- References:
Printable Strain Report