Strain JW4103-1
- CGSC#: 10954
- Strain Designation
Designation Source Preference JW4103-1 Keio Collection 1 JW4103 original Keio designation 2 - Sex(Hfr,F+,F-,or F'): F-
- 7 Mutations:
Mutation Location Certainty Display DE(araD-araB)567 1.40 1 Δ(araD-araB)567 lacZ4787(del)(::rrnB-3) 7.81 1 ΔlacZ4787(::rrnB-3) LAM- 17.40 1 λ- rph-1 82.20 1 rph-1 DE(rhaD-rhaB)568 88.16 1 Δ(rhaD-rhaB)568 groL768(del)::kan 94.17 1 ΔgroL768::kan hsdR514 98.74 1 hsdR514 - Comments:
- From the Keio Collection of single-gene knockouts constructed through a collaboration of the Inst. of Adv. Biosci. at Keio Univ., the Nara Inst. of Sci. and Technol., Japan and Purdue Univ., USA
- There is some question as to if this strain has a complete knockout of groL
- While this strain is reported to have a duplicate functioning groL gene, the expression is greatly reduced (see Aich 2011 reference)
- References:
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
- 2011. Plasmid-mediated transformation of Escherichia coli having a dependence on the heat-shock protein GroEL Int. J. Genet. Mol. Biol. 3(3):51-54.
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