E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pKD32

• Name: pKD32
• Type: Plasmid
  Plasmid Sites:

  oriR6Kgamma

  tL3LAM(Ter)

  bla(ApR)

  rgnB(Ter)

  cat

  
  
• Priority: 0
• Comment:
  • Like pKD13 (template plasmid), except CmR.
• References:
  • Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
  
  
  • Datsenko, K.A., B.L. Wanner 2003. unpublished results 200:3
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  BW25141/pKD32	11	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD32