E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Site pKD119

• Name: pKD119
• Type: Plasmid
  Plasmid Sites:

  repA101(ts)

  araBp-gam-bet-exo

  oriR101

  tetR

  
  
• Priority: 0
• Comment:
  • Like pKD46 (Lambda Red recombinase expression plasmid), except tetR. See pKD46
• References:
  • Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
  
  
  • Datsenko, K.A., B.L. Wanner 2003. unpublished results 200:3
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  BW25141/pKD119	11	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD119