E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pKD4

• Name: pKD4
• Type: Plasmid
  SubSites:

  bla

  FRT

  kan

  
  
  Plasmid Sites:

  oriR6Kgamma

  bla(ApR)

  rgnB(Ter)

  kan

  
  
• Priority: 0
• External Database Links:

  Host Site	Page Links
  NCBI/GenBank	AY048743
  CGSC	pKD4_Map.pdf

  
  
• Comment:
  • template plasmid for gene disruption. The resistance gene is flanked by FRT sites. 3267 basepairs. GenBk.Seq.AY048743.
• References:
  • Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  BW25141/pKD4	11	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD4