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Mutation galE15(GalS)

• Name: galE15(GalS)
• Type: Base Pair Substitution
• Mutation of Gene: galE
• Approx. Map Location: 17.03
• Synonyms

  Designation	Where Used
  CA3

  
  
• Properties

  Property	Symbol
  UDPGal accum.toxic if GalKRP+	GalS

  
  
• Comments
  • G to A transition resulting in an S122F amino acid substitution
  • is Brenner's gal^-_U38, isolated by UV mutagenesis from his strain CA1.
• References
  • Durfee, T, R Nelson, S Baldwin, G Plunkett, V Burland, B Mau, JF Petrosino, X Qin, DM Muzny, M Ayele, RA Gibbs, B Csorgo, G Posfai, GM Weinstock, FR Blattner 2008. The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190(7):2597-606.
• 52 Strains Carrying This Mutation

  Name	Mutations	Genotype
  CA3	5	Hfr(PO1), galE15(GalS), λ-, e14-, relA1, spoT1
  M26	6	F-, galE15(GalS), λ-, e14-, relA1, rpsL150(strR), spoT1
  N198	6	F', Δ(codB-lacI)3, galK16, galE15(GalS), relA1, rpsL150(strR), spoT1, F100-2
  M32	7	F-, galK16, galE15(GalS), λ-, e14-, relA1, rpsL150(strR), spoT1
  JS216	8	F-, DE9990, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, mcrA0
  M182	8	F-, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, relA1, rpsL150(strR), spoT1
  DF1062	11	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, relA1, rpsL150(strR), spoT1, zjf-920::Tn10
  MC1000	11	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, relA1, rpsL150(strR), spoT1, mcrB1?
  MC1060	11	F-, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0?, relA1, rpsL150(strR), spoT1, mcrB1?, hsdR2
  MC1060/pWTZ594	11	F-, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0?, relA1, rpsL150(strR), spoT1, mcrB1?, hsdR2, pWTZ594
  MPh42	11	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, relA1, rpsL150(strR), spoT1, mcrB1?
  RI89	12	F-, [araD139]B/r?, ΔaraBAD-714, Δ(codB-lacI)3, phoR82, galK16, galE15(GalS), λ-, e14-?, relA1, rpsL150(strR), spoT1, mcrB1?
  ALS225	13	F', Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB9999, hsdR2, F128-xxx
  ALS226	13	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB9999, hsdR2, F128-xx
  CG2	13	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, phoR82, secD1, galK16, galE15(GalS), λ-, e14-?, relA1, rpsL150(strR), spoT1, mcrB1?
  JS154	13	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, mcrA0, dicB2::Tn5, relA1, rpsL150(strR), spoT1, mcrB9999, hsdR2
  KJ185	13	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, phoR82, zaj-2085::Tn10, galK16, galE15(GalS), λ-, e14-?, relA1, rpsL150(strR), spoT1, mcrB1?
  MB2159	13	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, dadX100::FRT, relA1, rpsL150(strR), spoT1, alr-100::FRT, mcrB1
  MC1061	13	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB1, hsdR2
  MJAB2	13	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, mcrA0, relA1, rpsL150(strR), spoT1, rfaH564::Tn5, mcrB9999, hsdR2
  RI179	13	F-, [araD139]B/r?, ΔaraBAD-714, Δ(codB-lacI)3, phoR82, galK16, galE15(GalS), λ-, e14-?, relA1, dsbC3::Cm, rpsL150(strR), spoT1, mcrB1?
  RI242	13	F-, [araD139]B/r?, ΔaraBAD-714, Δ(codB-lacI)3, phoR82, galK16, galE15(GalS), λ-, e14-?, relA1, rpsL150(strR), spoT1, dipZ4::Cm, mcrB1?
  RI317	13	F-, [araD139]B/r?, ΔaraBAD-714, Δ(codB-lacI)3, phoR82, galK16, galE15(GalS), λ-, e14-?, dsbB2::kan, relA1, rpsL150(strR), spoT1, mcrB1?
  RI90	13	F-, [araD139]B/r?, ΔaraBAD-714, Δ(codB-lacI)3, phoR82, galK16, galE15(GalS), λ-, e14-?, relA1, rpsL150(strR), spoT1, dsbA1::kan, mcrB1?
  JS153	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, mcrA0, dicA1, dicB2::Tn5, relA1, rpsL150(strR), spoT1, mcrB9999, hsdR2
  K1471	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, mcrA0, relA1, rpoN208::Tn10, rpsL150(strR), spoT1, mcrB1, hsdR2
  K1472	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, mcrA0, Δ(pspA-pspC)637, relA1, rpsL150(strR), spoT1, mcrB1, hsdR2
  K1520	14	F-, Δ(araA-leu)7697, [araD139]B/r, galK16, galE15(GalS), λ-, e14-, mcrA0, Δ(pspA-pspC)637, relA1, rpoN208::Tn10, rpsL150(strR), spoT1, mcrB1, hsdR2
  KJ173	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, phoR82, zaj-2085::Tn10, secD29(Cs), galK16, galE15(GalS), λ-, e14-, relA1, rpsL150(strR), spoT1, mcrB1?
  KJ184	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, phoR82, zaj-2085::Tn10, secF62(Cs), galK16, galE15(GalS), λ-, e14-, relA1, rpsL150(strR), spoT1, mcrB1?
  MC1009	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?
  MC1009/pSL100	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL100
  MC1009/pSL102	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL102
  MC1009/pSL103	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL103
  MC1009/pSL105	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL105
  MC1009/pSL106	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL106
  MC1009/pSL107	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL107
  MC1009/pSL108	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL108
  MC1009/pSL109	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL109
  MC1009/pSL111	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL111
  MC1009/pSL112	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL112
  MC1009/pSL113	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL113
  MC1009/pSL114	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL114
  MC1009/pSL115	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL115
  MC1009/pSL117	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL117
  MC1009/pSL118	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-?, mcrA0, recA56, srlD300::Tn10, relA1, rpsL150(strR), spoT1, mcrB1?, pSL118
  SK6582	14	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, bolA2::kan, galK16, galE15(GalS), λ-, e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB9999, hsdR2
  VIP205	14	F-, Δ(araA-leu)7697, [araD139]B/r, Ωkan,T,lacIQ, P\_tac-ftsZ20, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, mcrA0, relA1, rpsL150(strR), mcrB9999, hsdR2
  WTZ18003	14	F-, Δ(araA-leu)7697, [araD139]B/r, ΔfhuA766::kan, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB1, hsdR2
  WTZ18005	14	F-, Δ(araA-leu)7697, [araD139]B/r, ΔfhuA866::FRT, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB1, hsdR2
  K1518	15	F-, Δ(araA-leu)7697, [araD139]B/r, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, mcrA0, pspF877::mini-Tn10, Δ(pspA-pspC)637, relA1, rpsL150(strR), spoT1, mcrB1, hsdR2
  WTZ18006	15	F-, Δ(araA-leu)7697, [araD139]B/r, ΔfhuA866::FRT, Δ(codB-lacI)3, galK16, galE15(GalS), λ-, e14-, mcrA0, relA1, rpsL150(strR), spoT1, ΔlamB832::FRT, mcrB1, hsdR2