E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Reviving Strains
• Contact Information

Mutation gapA12(del)::Cm

• Name: gapA12(del)::Cm
• Type: Tn
• Mutation of Gene: gapA
• Approx. Map Location: 40.09
• Properties

  Property	Symbol
  deletion	del

  
  
• Comments
  • : gap/epd deleted strains should be grown on minimal supplemented with casamino acids, Trp, Met, Thi, succinate + glycerol + antibiotic; they do not grow on LB or glucose media.
• References
  • Seta, F.D., S. Boschi-Muller, M.L. Vignais, G. Branlant 1997. Characterization of Escherichia coli strains with gapA and gapB genes deleted. J.Bacteriol. 179:5218-5221
• 4 Strains Carrying This Mutation

  Name	Mutations	Genotype
  DS112	3	F-, λ-, ΔgapA12::Cm, rph-1
  DS113	4	F-, λ-, ΔgapA12::Cm, Δepd-11::Ery, rph-1
  DS122	4	F-, λ-, ΔgapA12::Cm, IN(rrnD-rrnE)1, rph-1
  DS123	5	F-, λ-, ΔgapA12::Cm, Δepd-11::Ery, IN(rrnD-rrnE)1, rph-1