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CGSC, The Coli Genetic Stock Center

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Mutation thyA36

• Name: thyA36
• Type: Unknown
• Mutation of Gene: thyA
• Approx. Map Location: 63.86
• Comments
  • Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• References
• 29 Strains Carrying This Mutation

  Name	Mutations	Genotype
  P3478	5	F-, λ-, e14-, thyA36, IN(rrnD-rrnE)1, polA1(Am), deoC2
  W3110 Thy-	5	F-, λ-, thyA36, IN(rrnD-rrnE)1, rph-1, deoC2
  CBK103	6	F-, λ-, thyA36, IN(rrnD-rrnE)1, cysG98::Tn5, polA1(Am), deoC2
  CM4050	6	F-, lacZ53(Am), λ-, thyA36, rpsL151(strR), polA5, deoC2?
  DE418	6	F-, λ-?, thyA36?, IN(rrnD-rrnE)1?, rph-1?, rpoB331(rifR)?, hsdR19?, pLM2
  HL944	6	F-, λ-, thyA36, IN(rrnD-rrnE)1, rph-1, recQ1803::Tn3, deoC2
  HMS180	6	F-, λ-, thyA36, IN(rrnD-rrnE)1, rph-1?, rpoB331(rifR), hsdR19
  JG110	6	F-, λ-, thyA36, IN(rrnD-rrnE)1, metE70, polA5, deoC2
  CM4935	7	F-, lacZ53(Am), thyA36, rpsL151(strR), metE70, rha-5, deoC2?, λNM857
  MM384	7	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), rha-5, deoC2
  CM5255	8	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), zig-1863::Tn10, polA4113(ts), deoC3
  JG108	8	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), metE70, rha-5, deoC2
  JG137	8	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), rha-5, uvrA6, deoC2?
  JW165	8	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), rha-5, malB45, deoC2
  KLC438	8	F-, λ-, thyA36, IN(rrnD-rrnE)1, rha-8, melA7, amp-50, deoC2, met-184?
  MM383	8	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), polA12(ts), rha-5, deoC2
  ES1301	9	F-, lacZ53(Am), λ-, mutS201::Tn5, thyA36, IN(rrnD-rrnE)1, rha-5, metB1, deoC2?, pur-?
  ES1481	9	F-, lacZ53(Am), λ-, mutS215::Tn10, thyA36, IN(rrnD-rrnE)1?, rha-5, metB1, deoC2, pur-
  ES1484	9	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rha-5, metB1, mutL218::Tn10, deoC2?, pur-
  HMS85	9	F-, polB100, leu-85, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), deoC2?, lys-34
  JG123	9	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), metE70, rha-5, malB45, deoC2
  JW164	9	F-, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), polA1(Am), rha-5, malB45, deoC2
  JW164	9	F-, lacZ53(Am), λ-, e14-, thyA36, rpsL151(strR), polA1(Am), rha-5, malB45, deoC2
  KLC436	9	F-, λ-, thyA36, IN(rrnD-rrnE)1, rha-8, ssb-1(ts), melA7, amp-50, deoC2, met-184
  SR1059	9	F-, leuB19, lacZ53(Am), bioA2, thyA36, rpsL151(strR), metE70, rha-5, malB45, deoC2
  SR645	9	F-, leuB19, lacZ53(Am), uvrB230?, thyA36, rpsL151(strR), metE70, rha-5, uvrA6, deoC2
  HMS83	10	F-, polB100, lacZ53(Am), λ-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), polA1(Am), rha-5, deoC2?, lys-34
  SR1060	10	F-, leuB19, lacZ53(Am), bioA2, recN2001, thyA36, rpsL151(strR), metE70, rha-5, malB45, deoC2
  SR350	10	F-, leuB19, lacZ53(Am), phr-2, uvrB230, thyA36, rpsL151(strR), metE70, rha-5, uvrA6, deoC2