CGSC Strain#: 6361
Strain Designation: NK6069      Source of Strain: N. Kleckner
Sex: F- Chromosomal Markers: pro-82, glnX44(AS), λ^-, endA1, thiE1, purD81(KanR)::Tn5, hsdR17
Strain Comments:

• This is a derivative of strain MM294A.
• This strain requires histidine for growth on supplemented minimal medium (and addition of methionine as well enhances growth).
• pro-82-- is in proA or proB.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• = kanamycin resistant
• AS = amber(UAG) suppressor
• KanR = kanamycin resistance

References: