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Strain BW18920

• CGSC#: 8172
• Strain Designation

  Designation	Source	Preference
  BW18920	Wanner B.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 12 Mutations:

  Mutation	Location	Certainty	Display
  araBAD-0	1.40	1	araBAD-0
  DE(argF-lac)169	6.21	1	Δ(argF-lac)169
  phoA532(del)	8.64	1	ΔphoA532
  tsx-0	9.28	1	tsx-0
  trpA9605(Am)	28.33	1	trpA9605(Am)
  DE(dgsA-manA)726	35.88	1	Δ(dgsA-manA)726
  zdg-232::Tn10	36.10	1	zdg-232::Tn10
  his-85(Am)	45.01	0	his-85(Am)
  IN(rrnD-rrnE)1	73.74	1	IN(rrnD-rrnE)1
  hsdR514	98.74	1	hsdR514
  creC510	99.89	1	creC510
  ilv-1	1000.00	1	ilv-1

  
  
• Comments:
  • his-85(Am) was annotated in this strain as his-29(Am), but we are not sure that the original his-29 is an amber mutation, and we are speculating that the amber allele shown is the one in this strain.
• References:
  • Metcalf, W.W., W. Jiang, B.L. Wanner 1994. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6Kgamma origin plasmids at different copy numbers. Gene 138:1-7
  
  

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